Immune Repertoire Profiling with DNBSEQ

Immune Repertoire Profiling with DNBSEQ

Immune Repertoire Profiling with DNBSEQ

Immune Repertoire Profiling with DNBSEQ

Immune Repertoire Profiling with DNBSEQ

Oct 26, 2023

Oct 26, 2023

Immune Repertoire Profiling with DNBSEQ
Immune Repertoire Profiling with DNBSEQ
Immune Repertoire Profiling with DNBSEQ
Immune Repertoire Profiling with DNBSEQ
Dr. Sean Huang - Director, Lab & Services - Precision Genomics @MiRXES
Dr. Sean Huang - Director, Lab & Services - Precision Genomics @MiRXES
Dr. Sean Huang - Director, Lab & Services - Precision Genomics @MiRXES
Dr. Sean Huang - Director, Lab & Services - Precision Genomics @MiRXES

Dr. Sean Huang

Dr. Sean Huang

Dr. Sean Huang

Dr. Sean Huang

Director, Lab & Services

Director, Lab & Services

Director, Lab & Services

Director, Lab & Services

Dr. Yang Heng - Scientist @BGI Research
Dr. Yang Heng - Scientist @BGI Research
Dr. Yang Heng - Scientist @BGI Research
Dr. Yang Heng - Scientist @BGI Research

Dr. Yang Heng

Dr. Yang Heng

Dr. Yang Heng

Dr. Yang Heng

Scientist @BGI Research

Scientist @BGI Research

Scientist @BGI Research

Scientist @BGI Research

Dr. Xiaojing Huo - Senior Product Manager @Singleron Biotechnologies
Dr. Xiaojing Huo - Senior Product Manager @Singleron Biotechnologies
Dr. Xiaojing Huo - Senior Product Manager @Singleron Biotechnologies
Dr. Xiaojing Huo - Senior Product Manager @Singleron Biotechnologies

Dr. Xiaojing Huo

Dr. Xiaojing Huo

Dr. Xiaojing Huo

Dr. Xiaojing Huo

Senior Product Manager @Singleron Biotechnologies

Senior Product Manager @Singleron Biotechnologies

Senior Product Manager @Singleron Biotechnologies

Senior Product Manager @Singleron Biotechnologies

Dr. John Beckford - Scientist @Takara Bio
Dr. John Beckford - Scientist @Takara Bio
Dr. John Beckford - Scientist @Takara Bio
Dr. John Beckford - Scientist @Takara Bio

Dr. John Beckford

Dr. John Beckford

Dr. John Beckford

Dr. John Beckford

Scientist @Takara Bio

Scientist @Takara Bio

Scientist @Takara Bio

Scientist @Takara Bio

Dr. Shaveta Goyal - Staff Scientist @Takara Bio
Dr. Shaveta Goyal - Staff Scientist @Takara Bio
Dr. Shaveta Goyal - Staff Scientist @Takara Bio
Dr. Shaveta Goyal - Staff Scientist @Takara Bio

Dr. Shaveta Goyal

Dr. Shaveta Goyal

Dr. Shaveta Goyal

Dr. Shaveta Goyal

Staff Scientist @Takara Bio

Staff Scientist @Takara Bio

Staff Scientist @Takara Bio

Staff Scientist @Takara Bio

Immune Repertoire Profiling with DNBSEQ

In the world of genomics and biomedical research, the ability to accurately sequence and analyze genetic material is paramount. Researchers rely on cutting-edge sequencing technologies to unlock the secrets of DNA and RNA, paving the way for groundbreaking discoveries in fields such as disease research, oncology, and more. In this blog post, we'll dive into the transformative capabilities of MGI sequencers, specifically the DNBSEQ-G99, T7, and G400, as highlighted in the recent webinar on "Immune Repertoire Profiling with DNBSEQ."

MGI Sequencers: Empowering Genomic Research

Before we delve into the details of the webinar, let's take a moment to understand the significance of MGI sequencers in the realm of genomics. MGI, a subsidiary of BGI Group, is renowned for its cutting-edge sequencing technologies that have been instrumental in advancing genomic research worldwide. Their sequencers are known for their precision, scalability, and cost-effectiveness, making them ideal choices for a wide range of applications.

In the field of immune repertoire profiling and beyond, MGI sequencers have proven to be invaluable tools, offering researchers the ability to generate high-quality data and gain deeper insights into complex biological systems. Now, let's explore the highlights from the webinar and how MGI sequencers play a pivotal role in the presented research.

Immune Repertoire Profiling: A Window into Disease Research

The webinar titled "Immune Repertoire Profiling with DNBSEQ" takes us on a journey into the world of T-cell receptor (TCR) and B-cell receptor (BCR) sequencing. Immune repertoire profiling, a fundamental aspect of immunogenetics, plays a crucial role in understanding the immune system's response to diseases and pathogens. The ability to sequence and analyze TCRs and BCRs with high precision is essential for unraveling the complexities of immune responses.

The webinar's focus is on the innovative methodology used to capture, sequence, and analyze immune repertoires, shedding light on the powerful capabilities of MGI sequencers, particularly the DNBSEQ-G99, T7, and G400. Let's explore the key takeaways from the webinar:

DNBSEQ Technology: A Game-Changer

The webinar introduces us to DNBSEQ technology, which forms the foundation of MGI sequencers. DNBSEQ, or DNA Nanoball Sequencing, is a revolutionary approach that offers several advantages:

  1. Precise Immune Profiling: DNBSEQ technology enables precise immune profiling, a critical aspect of disease research and oncology studies. It allows researchers to capture and sequence TCRs and BCRs with unmatched accuracy.

  2. Large Field of View: One of the standout features of DNBSEQ technology is its ability to offer a vast field of view. With large DNB chips measuring up to 13 by 13 cm, researchers can analyze extensive tissue sections, potentially encompassing half of the human brain.

  3. High Spatial Resolution: DNBSEQ technology boasts exceptional spatial resolution. With a center-to-center distance between spots of around 500 nanometers, researchers can achieve sub-cellular resolution, ensuring that gene expression data is not mixed from neighboring spots.

  4. Broad Applications: The versatility of DNBSEQ technology is evident from its applicability across various species, including mouse embryos, axolotls, human tissues, Mala cells, and more. This versatility has led to high-impact publications in prestigious journals like Cell, Nature, and Science (S).

Multiomic Insights with Stereo-Seq: RNA and Protein Together

While gene expression and transcriptomics provide valuable insights, they represent just one layer of information. To gain a comprehensive understanding of cellular identity and function, researchers need to combine different modalities, including genomic, epigenomic, and proteomic information.

The Power of Multiomic Integration

The webinar emphasizes the importance of integrating multiple data modalities. By combining genomic and proteomic information, researchers can accurately phenotype cell identities, uncover biological insights, and address complex questions in disease research.

Immune Repertoire Profiling with DNBSEQ

In the world of genomics and biomedical research, the ability to accurately sequence and analyze genetic material is paramount. Researchers rely on cutting-edge sequencing technologies to unlock the secrets of DNA and RNA, paving the way for groundbreaking discoveries in fields such as disease research, oncology, and more. In this blog post, we'll dive into the transformative capabilities of MGI sequencers, specifically the DNBSEQ-G99, T7, and G400, as highlighted in the recent webinar on "Immune Repertoire Profiling with DNBSEQ."

MGI Sequencers: Empowering Genomic Research

Before we delve into the details of the webinar, let's take a moment to understand the significance of MGI sequencers in the realm of genomics. MGI, a subsidiary of BGI Group, is renowned for its cutting-edge sequencing technologies that have been instrumental in advancing genomic research worldwide. Their sequencers are known for their precision, scalability, and cost-effectiveness, making them ideal choices for a wide range of applications.

In the field of immune repertoire profiling and beyond, MGI sequencers have proven to be invaluable tools, offering researchers the ability to generate high-quality data and gain deeper insights into complex biological systems. Now, let's explore the highlights from the webinar and how MGI sequencers play a pivotal role in the presented research.

Immune Repertoire Profiling: A Window into Disease Research

The webinar titled "Immune Repertoire Profiling with DNBSEQ" takes us on a journey into the world of T-cell receptor (TCR) and B-cell receptor (BCR) sequencing. Immune repertoire profiling, a fundamental aspect of immunogenetics, plays a crucial role in understanding the immune system's response to diseases and pathogens. The ability to sequence and analyze TCRs and BCRs with high precision is essential for unraveling the complexities of immune responses.

The webinar's focus is on the innovative methodology used to capture, sequence, and analyze immune repertoires, shedding light on the powerful capabilities of MGI sequencers, particularly the DNBSEQ-G99, T7, and G400. Let's explore the key takeaways from the webinar:

DNBSEQ Technology: A Game-Changer

The webinar introduces us to DNBSEQ technology, which forms the foundation of MGI sequencers. DNBSEQ, or DNA Nanoball Sequencing, is a revolutionary approach that offers several advantages:

  1. Precise Immune Profiling: DNBSEQ technology enables precise immune profiling, a critical aspect of disease research and oncology studies. It allows researchers to capture and sequence TCRs and BCRs with unmatched accuracy.

  2. Large Field of View: One of the standout features of DNBSEQ technology is its ability to offer a vast field of view. With large DNB chips measuring up to 13 by 13 cm, researchers can analyze extensive tissue sections, potentially encompassing half of the human brain.

  3. High Spatial Resolution: DNBSEQ technology boasts exceptional spatial resolution. With a center-to-center distance between spots of around 500 nanometers, researchers can achieve sub-cellular resolution, ensuring that gene expression data is not mixed from neighboring spots.

  4. Broad Applications: The versatility of DNBSEQ technology is evident from its applicability across various species, including mouse embryos, axolotls, human tissues, Mala cells, and more. This versatility has led to high-impact publications in prestigious journals like Cell, Nature, and Science (S).

Multiomic Insights with Stereo-Seq: RNA and Protein Together

While gene expression and transcriptomics provide valuable insights, they represent just one layer of information. To gain a comprehensive understanding of cellular identity and function, researchers need to combine different modalities, including genomic, epigenomic, and proteomic information.

The Power of Multiomic Integration

The webinar emphasizes the importance of integrating multiple data modalities. By combining genomic and proteomic information, researchers can accurately phenotype cell identities, uncover biological insights, and address complex questions in disease research.

Immune Repertoire Profiling with DNBSEQ

In the world of genomics and biomedical research, the ability to accurately sequence and analyze genetic material is paramount. Researchers rely on cutting-edge sequencing technologies to unlock the secrets of DNA and RNA, paving the way for groundbreaking discoveries in fields such as disease research, oncology, and more. In this blog post, we'll dive into the transformative capabilities of MGI sequencers, specifically the DNBSEQ-G99, T7, and G400, as highlighted in the recent webinar on "Immune Repertoire Profiling with DNBSEQ."

MGI Sequencers: Empowering Genomic Research

Before we delve into the details of the webinar, let's take a moment to understand the significance of MGI sequencers in the realm of genomics. MGI, a subsidiary of BGI Group, is renowned for its cutting-edge sequencing technologies that have been instrumental in advancing genomic research worldwide. Their sequencers are known for their precision, scalability, and cost-effectiveness, making them ideal choices for a wide range of applications.

In the field of immune repertoire profiling and beyond, MGI sequencers have proven to be invaluable tools, offering researchers the ability to generate high-quality data and gain deeper insights into complex biological systems. Now, let's explore the highlights from the webinar and how MGI sequencers play a pivotal role in the presented research.

Immune Repertoire Profiling: A Window into Disease Research

The webinar titled "Immune Repertoire Profiling with DNBSEQ" takes us on a journey into the world of T-cell receptor (TCR) and B-cell receptor (BCR) sequencing. Immune repertoire profiling, a fundamental aspect of immunogenetics, plays a crucial role in understanding the immune system's response to diseases and pathogens. The ability to sequence and analyze TCRs and BCRs with high precision is essential for unraveling the complexities of immune responses.

The webinar's focus is on the innovative methodology used to capture, sequence, and analyze immune repertoires, shedding light on the powerful capabilities of MGI sequencers, particularly the DNBSEQ-G99, T7, and G400. Let's explore the key takeaways from the webinar:

DNBSEQ Technology: A Game-Changer

The webinar introduces us to DNBSEQ technology, which forms the foundation of MGI sequencers. DNBSEQ, or DNA Nanoball Sequencing, is a revolutionary approach that offers several advantages:

  1. Precise Immune Profiling: DNBSEQ technology enables precise immune profiling, a critical aspect of disease research and oncology studies. It allows researchers to capture and sequence TCRs and BCRs with unmatched accuracy.

  2. Large Field of View: One of the standout features of DNBSEQ technology is its ability to offer a vast field of view. With large DNB chips measuring up to 13 by 13 cm, researchers can analyze extensive tissue sections, potentially encompassing half of the human brain.

  3. High Spatial Resolution: DNBSEQ technology boasts exceptional spatial resolution. With a center-to-center distance between spots of around 500 nanometers, researchers can achieve sub-cellular resolution, ensuring that gene expression data is not mixed from neighboring spots.

  4. Broad Applications: The versatility of DNBSEQ technology is evident from its applicability across various species, including mouse embryos, axolotls, human tissues, Mala cells, and more. This versatility has led to high-impact publications in prestigious journals like Cell, Nature, and Science (S).

Multiomic Insights with Stereo-Seq: RNA and Protein Together

While gene expression and transcriptomics provide valuable insights, they represent just one layer of information. To gain a comprehensive understanding of cellular identity and function, researchers need to combine different modalities, including genomic, epigenomic, and proteomic information.

The Power of Multiomic Integration

The webinar emphasizes the importance of integrating multiple data modalities. By combining genomic and proteomic information, researchers can accurately phenotype cell identities, uncover biological insights, and address complex questions in disease research.

DNBSEQ-G99
DNBSEQ-G99
DNBSEQ-G99

DNBSEQ-G99

Advancing Genomic Insights in Immune Profiling

The DNBSEQ-G99 from MGI stands out in immune repertoire profiling, offering unmatched precision and efficiency. Its sequencing prowess is key to accurately analyzing T-cell and B-cell receptors, enhancing our understanding of the immune system's role in diseases and potential therapeutic pathways.

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Stereo-Seq: Simultaneous RNA and Protein Detection

MGI's Stereo-Seq method is introduced as a groundbreaking approach to simultaneously detect gene expression (RNA) and protein expression on the same tissue section. Here's how it works:

  1. High-Plex Antibody Panels: Stereo-Seq leverages high-plex antibody panels, such as the 268 antibodies from B legend, to capture protein expression. These antibodies are designed with specific features, including adapters for PCR amplification and 15-nucleotide barcodes for antibody identification.

  2. Poly-A Tails and DNA Barcodes: The technology utilizes poly-A tails on the antibodies, along with DNA barcodes on the DNB chips, to capture both RNA and antibody-derived molecules in a single step.

  3. Library Construction and Sequencing: After capture, the process continues with library construction and sequencing, resulting in the generation of both RNA and protein profiles from the same tissue section.

  4. Unveiling Cellular Complexity: The power of Stereo-Seq lies in its ability to reveal the intricate details of cellular complexity. By examining the spatial distribution of gene expression and protein expression, researchers can differentiate cell subtypes and gain deeper insights into biological processes.

Data Consistency and Accuracy

Stereo-Seq's ability to provide consistent and accurate data is a critical factor in its utility for researchers. The webinar presents compelling evidence of data reproducibility and reliability:

  1. Consistency Across Sections: Researchers conducted experiments using three consecutive tissue sections, applying Stereo-Seq to each of them. The resulting gene expression and protein expression data exhibited high consistency and correlation, demonstrating the method's reproducibility.

  2. Correlation Between RNA and Protein: To assess accuracy, researchers compared protein expression patterns obtained through Stereo-Seq with immunofluorescence patterns. The strong correlation observed indicates the method's accuracy in reflecting true tissue biology.

  3. Plexity and Data Quality: Stereo-Seq's ability to handle different levels of plexity (i.e., the number of antibodies used) was tested. The results showed that increasing plexity did not negatively impact RNA or protein detection, highlighting the method's robustness.

Unlocking Insights Across Tissue Types

The webinar underscores Stereo-Seq's versatility by showcasing its application across various tissue types:

  1. Mouse Tissues: Researchers generated data from mouse tissues, revealing the spatial distribution of immune cells, microphages, red blood cells, and more. The sub-cellular resolution provided valuable insights into cellular interactions and structures.

  2. Human Tissues: Stereo-Seq's application extended to human tissues, where it distinguished different cell subtypes within the same tissue sections. The spatial distribution of cell markers highlighted the technology's potential in understanding complex human biology.

Stereo-Seq and the Integration of ADT Data

One intriguing aspect discussed in the webinar is the integration of Antibody-Derived Tag (ADT) data. Researchers compared ADT and mRNA data, revealing some interesting findings:

  • ADT Offers Unique Insights: ADT data, representing protein expression, showed significant differences compared to mRNA data. In some cases, proteins that were barely detected in transcriptomic data were clearly observed in the protein expression data.

  • Protein Contribution to Subtyping: Integrating ADT data contributed significantly to the identification of cell subtypes, both in gene expression and spatial context. This highlights the importance of including protein information in the analysis to gain a more comprehensive understanding of cell biology.

Future Directions: Harnessing Multiomic Integration

As researchers continue to explore the potential of Stereo-Seq and multiomic integration, exciting avenues for future research emerge. Here are some key points of interest:

  • Protein Degradation: Understanding protein degradation mechanisms and their role in diseases like Alzheimer's could open up new avenues for therapeutic interventions. Stereo-Seq's ability to capture protein expression may play a pivotal role in such investigations.

  • Discrepancies in Protein and mRNA Data: Further research is needed to elucidate the reasons behind discrepancies observed between protein and mRNA data. Investigating why certain genes show discordant expression patterns could yield valuable insights.

  • Cell Subtyping: Leveraging integrated multiomic data to identify and classify cell subtypes with precision holds great promise. This could lead to breakthroughs in personalized medicine and targeted therapies.

  • Disease Biomarker Discovery: The combination of RNA and protein data offers a powerful platform for discovering disease biomarkers. Identifying specific molecules associated with diseases can aid in early diagnosis and treatment strategies.

Conclusion: MGI Sequencers Transform Genomic Research

In conclusion, the webinar on "Immune Repertoire Profiling with DNBSEQ" showcases the remarkable capabilities of MGI sequencers, particularly the DNBSEQ-G99, T7, and G400, in advancing genomic research. The integration of multiomic data through Stereo-Seq opens up new possibilities for understanding complex biological systems, disease mechanisms, and cellular interactions.

MGI sequencers have emerged as indispensable tools for researchers in their quest to unlock the secrets of the genome. Whether in the study of immune repertoires, disease research, or personalized medicine, the precision and versatility of MGI sequencers are driving discoveries that have the potential to reshape the future of healthcare.

With the ability to capture both RNA and protein data simultaneously, researchers can delve deeper into the molecular intricacies of cells and tissues. This comprehensive approach promises to accelerate progress in fields as diverse as immunology, oncology, neuroscience, and beyond.

As the field of genomics continues to evolve, MGI sequencers stand at the forefront, empowering researchers to push the boundaries of knowledge and drive innovation. The future of genomic research is bright, and MGI sequencers are illuminating the way.

Disclaimer: The information provided in this blog is for educational and informational purposes only and is not intended for use in diagnostic procedures. MGI products mentioned are for research use only.

DNBSEQ-T7
DNBSEQ-T7
DNBSEQ-T7

DNBSEQ-T7

Precision in Immune Repertoire Profiling

MGI's DNBSEQ-T7 is a vital tool for immune repertoire profiling in genomics and biomedical research. With its high-throughput sequencing capabilities, the T7 is adept at capturing intricate genetic variations, crucial for understanding immune responses and advancing fields like oncology and disease research.

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pagination here
pagination here

Genomics

Sequencing Technology

Immune Repertoire Profiling

MGI Sequencers

Multiomic Integration

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Join our newsletter to stay up to date on our latest webinars.

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Copyright © 2024 MGI tech GmbH, Ltd. All Rights Reserved.

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Join our newsletter to stay up to date on our latest webinars.

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Copyright © 2024 MGI tech GmbH, Ltd. All Rights Reserved.